Hotspot

rpl16-rps3

LSC · 82759-83860 · 1102 bp

Back to Illigera

Pi 0.0104

MarkerSeek score 67.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 17
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	CCCGAACTCTCTATGGGGTA	50.0	55.0	58.0	57.6	1271-1276 bp	1.0	79.1
rpl16-rps3_p2	GAAGAGCGTATCTGCCGAAA	CCCGAACTCTCTATGGGGTA	50.0	55.0	58.1	57.6	1262-1267 bp	1.0	78.6
rpl16-rps3_p3	AACAAATACGATTGCCCCGA	CCCGAACTCTCTATGGGGTA	45.0	55.0	57.9	57.6	1244-1249 bp	1.0	78.4
rpl16-rps3_p4	AGCGTATCTGCCGAAACAAA	CCCGAACTCTCTATGGGGTA	45.0	55.0	58.2	57.6	1258-1263 bp	1.0	77.7
rpl16-rps3_p5	TGTTTGCGGAATCTGGTTCT	CCCGAACTCTCTATGGGGTA	45.0	55.0	57.7	57.6	1194-1199 bp	1.0	77.2