Hotspot

trnP-UGG-psaJ

LSC · 68067-68423 · 357 bp

Back to Illigera

Pi 0.0187

MarkerSeek score 65.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 10
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CTATCAATAGGCCCGCCAAA	50.0	50.0	58.1	57.7	494-496 bp	1.0	78.7
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	TATCAATAGGCCCGCCAAAG	50.0	50.0	58.1	57.7	493-495 bp	1.0	78.7
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	TATCAATAGGCCCGCCAAAG	50.0	50.0	58.1	57.7	492-494 bp	1.0	78.7
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	CTATCAATAGGCCCGCCAAA	50.0	50.0	58.1	57.7	493-495 bp	1.0	78.6
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TATCAATAGGCCCGCCAAAG	50.0	50.0	58.1	57.7	504-506 bp	1.0	78.6