Hotspot

psbE-petL

LSC · 66171-67262 · 1092 bp

Back to Illigera

Pi 0.0167

MarkerSeek score 64.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 26
pi: 0.0167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.3	57.1	1196-1211 bp	1.0	70.6
psbE-petL_p2	CAGCAAAAGAACGTTCTCCC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.3	57.1	1199-1214 bp	1.0	70.4
psbE-petL_p3	AGCAAAAGAACGTTCTCCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.8	57.1	1198-1213 bp	1.0	69.7
psbE-petL_p4	CAAAAGAACGTTCTCCCGTG	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.3	57.8	1196-1211 bp	1.0	68.3
psbE-petL_p5	CAAAAGAACGTTCTCCCGTG	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.3	56.8	1197-1212 bp	1.0	68.2