Hotspot

trnE-UUC-trnT-GGU

LSC · 32026-32700 · 675 bp

Back to Illigera

Pi 0.0138

MarkerSeek score 67.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 14
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	779-784 bp	1.0	80.1
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	758-763 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TAAGACTGGAGCTTCGTGGA	50.0	50.0	57.8	58.1	819-824 bp	1.0	79.5
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	755-760 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	GACTGGAGCTTCGTGGAAAA	50.0	50.0	57.8	58.1	816-821 bp	1.0	79.1