Hotspot

trnS-GCU-trnG-UCC

LSC · 8881-9673 · 793 bp

Back to Illigera

Pi 0.018

MarkerSeek score 61.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 21
pi: 0.018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGTCGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	854-862 bp	1.0	55.0
trnS-GCU-trnG-UCC_p2	AATCCGTCGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	853-861 bp	1.0	55.0
trnS-GCU-trnG-UCC_p3	CAATCCGTCGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	857-865 bp	1.0	54.0
trnS-GCU-trnG-UCC_p4	CAATCCGTCGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	856-864 bp	1.0	54.0
trnS-GCU-trnG-UCC_p5	AATCCGTCGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	855-863 bp	1.0	54.0