Hotspot

rps16-trnQ-UUG

LSC · 6022-7599 · 1578 bp

Back to Illigera

Pi 0.0133

MarkerSeek score 64.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1578
variable_sites: 31
pi: 0.0133
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCAAGAATCCACAGCCGATA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1680-1757 bp	1.0	82.0
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1651-1728 bp	1.0	81.3
rps16-trnQ-UUG_p3	CCACATCGCTTCAAACGAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1631-1708 bp	1.0	80.6
rps16-trnQ-UUG_p4	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1672-1749 bp	1.0	79.7
rps16-trnQ-UUG_p5	ACCACATCGCTTCAAACGAA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	1632-1709 bp	1.0	79.2