Hotspot

ndhA

Genome · 118433-120598 · 2166 bp

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Pi 0.00339

MarkerSeek score 59.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2166
variable_sites: 27
pi: 0.00339
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GCATAGGGAACATATCGTGGA	GCAAATTCTTCCTCAGCTGG	47.6	50.0	57.3	57.1	2336-2394 bp	1.0	61.2
ndhA_p2	AGCATAGGGAACATATCGTGG	GCAAATTCTTCCTCAGCTGG	47.6	50.0	57.3	57.1	2337-2395 bp	1.0	61.1
ndhA_p3	TCCATTCAGCATAGGGAACA	GCAAATTCTTCCTCAGCTGG	45.0	50.0	56.2	57.1	2344-2402 bp	1.0	60.2
ndhA_p4	TCTCTTGCTTCAGAGAGGTT	GCAAATTCTTCCTCAGCTGG	45.0	50.0	55.8	57.1	2289-2347 bp	1.0	57.0
ndhA_p5	GCATAGGGAACATATCGTGGAT	GCAAATTCTTCCTCAGCTGG	45.5	50.0	57.8	57.1	2336-2394 bp	1.0	56.9