Hotspot

trnV-GAC-rps12

Genome · 137032-138804 · 1773 bp

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Pi 0.00404

MarkerSeek score 55.90

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: Genome
length_bp: 1773
variable_sites: 21
pi: 0.00404
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.9	57.7	1879-2059 bp	0.733333	71.0
trnV-GAC-rps12_p2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1818-1998 bp	0.733333	70.8
trnV-GAC-rps12_p3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1822-2002 bp	0.733333	70.7
trnV-GAC-rps12_p4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1817-1997 bp	0.733333	70.7
trnV-GAC-rps12_p5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.3	57.7	1820-2000 bp	0.733333	69.8