Hotspot

psaC-ndhE

Genome · 116055-116339 · 285 bp

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Pi 0.0523

MarkerSeek score 61.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: Genome
length_bp: 285
variable_sites: 33
pi: 0.0523
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAGGCTCGTACACATTGAGT	TCAACTCGTATCAATCAATCT	50.0	33.3	57.6	52.4	381-386 bp	1.0	48.1
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	TCAACTCGTATCAATCAATCT	55.0	33.3	58.3	52.4	397-402 bp	1.0	47.8
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	TCAACTCGTATCAATCAATCT	55.0	33.3	58.3	52.4	398-403 bp	1.0	47.8
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	TCAACTCGTATCAATCAATCT	50.0	33.3	58.1	52.4	421-426 bp	1.0	47.4
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	TCAACTCGTATCAATCAATCT	50.0	33.3	57.6	52.4	427-432 bp	1.0	47.3