Hotspot

psbF-psbE

Genome · 67699-67707 · 9 bp

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Pi 0.0275

MarkerSeek score 58.60

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: Genome
length_bp: 9
variable_sites: 1
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	AACAGCCAACCATCTCACTG	ATAACTGGCCGTTTCGATCC	50.0	50.0	58.1	58.1	117 bp	1.0	79.5
psbF-psbE_p2	GTGAACAGCCAACCATCTCA	ATAACTGGCCGTTTCGATCC	50.0	50.0	58.1	58.1	120 bp	1.0	79.4
psbF-psbE_p3	TGAACAGCCAACCATCTCAC	ATAACTGGCCGTTTCGATCC	50.0	50.0	58.1	58.1	119 bp	1.0	79.4
psbF-psbE_p4	AACAGCCAACCATCTCACTG	ACCGACAGGGGATTCCATTA	50.0	50.0	58.1	58.1	137 bp	1.0	78.9
psbF-psbE_p5	TGAACAGCCAACCATCTCAC	ACCGACAGGGGATTCCATTA	50.0	50.0	58.1	58.1	139 bp	1.0	78.8