Hotspot

trnF-GAA-ndhJ

Genome · 51019-51669 · 651 bp

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Pi 0.00422

MarkerSeek score 60.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 651
variable_sites: 10
pi: 0.00422
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	578-830 bp	1.0	84.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	542-794 bp	1.0	84.3
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	566-818 bp	1.0	83.6
trnF-GAA-ndhJ_p4	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	588-840 bp	1.0	83.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGCCTGAAAGTTGGATCGG	55.0	50.0	58.0	58.2	555-807 bp	1.0	83.2