Hotspot

trnK-UUU-rps16

Genome · 4878-5680 · 803 bp

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Pi 0.00521

MarkerSeek score 60.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: Genome
length_bp: 803
variable_sites: 13
pi: 0.00521
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGAGCTCAACCTACAGGAAC	50.0	55.0	58.2	57.6	911-936 bp	1.0	79.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGAGCTCAACCTACAGGAAC	50.0	55.0	58.2	57.6	910-935 bp	1.0	79.5
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	GGAGCTCAACCTACAGGAAC	50.0	55.0	58.3	57.6	920-945 bp	1.0	78.5
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGAGGT	50.0	45.0	58.2	57.4	881-906 bp	1.0	78.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGAGGT	50.0	45.0	58.2	57.4	880-905 bp	1.0	78.0