primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGAGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	57.6	20.3	17.2	0.556730	4878	5680	911-936	1.000000	79.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGAGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	57.6	20.3	17.2	0.556730	4878	5680	910-935	1.000000	79.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	GGAGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.3	57.6	5.3	17.2	0.671116	4878	5680	920-945	1.000000	78.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAGGCGGAGGT	20	20	50.0	45.0	58.2	57.4	20.3	0.0	0.755068	4878	5680	881-906	1.000000	78.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAGGCGGAGGT	20	20	50.0	45.0	58.2	57.4	20.3	0.0	0.755068	4878	5680	880-905	1.000000	78.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ATCATGTCGTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.9	58.1	0.0	16.9	0.154950	6779	8352	1653-1765	1.000000	84.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6779	8352	1641-1753	1.000000	84.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	ATCATGTCGTTCAAGTCGCA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	57.9	57.8	0.0	16.9	0.290201	6779	8352	1657-1769	1.000000	83.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6779	8352	1645-1757	1.000000	83.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCGTTCAAGTCGCA	CGGTAAGGCAACAGGTTTTG	20	20	45.0	50.0	57.9	57.6	0.0	0.0	0.494546	6779	8352	1686-1798	1.000000	81.9
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GAATTAGCCTGGGACGGAAG	GGGCAATTTGGCGAAGAAAA	20	20	55.0	45.0	58.0	57.8	0.0	15.8	0.207392	8425	8764	491-511	1.000000	84.7
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GAAGGATTCGAACCTCCGAG	GGGCAATTTGGCGAAGAAAA	20	20	55.0	45.0	57.8	57.8	16.4	15.8	0.373672	8425	8764	475-495	1.000000	83.6
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	CAAAACCTGTTGCCTTACCG	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	57.6	57.8	0.0	15.8	0.578017	8425	8764	446-466	1.000000	82.4
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	AGGATTCGAACCTCCGAGTA	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	57.6	57.8	11.3	15.8	0.594273	8425	8764	473-493	1.000000	81.9
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	AGAATTAGCCTGGGACGGAA	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	58.4	57.8	0.0	15.8	0.582260	8425	8764	492-512	1.000000	81.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	57.6	57.7	0.9	0.0	2.711020	9690	10581	968-1085	1.000000	65.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGATCCCT	20	22	55.0	40.9	58.5	57.7	0.0	0.0	2.817034	9690	10581	963-1080	1.000000	64.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TAGTCCACTCAGCCATCTCT	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	57.2	57.7	0.0	0.0	3.137708	9690	10581	964-1081	1.000000	61.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGATCCC	20	21	50.0	42.9	57.6	56.2	0.9	0.0	3.179768	9690	10581	968-1085	1.000000	61.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGATCCC	20	21	55.0	42.9	58.5	56.2	0.0	0.0	3.285782	9690	10581	963-1080	1.000000	60.4
petN-psbM_p1	petN-psbM	1	CTTGGGCGGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	9.6	0.0	1.649741	30871	31883	1147-1163	1.000000	68.7
petN-psbM_p2	petN-psbM	2	TTGGGCGGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	9.6	0.0	1.649741	30871	31883	1146-1162	1.000000	68.7
petN-psbM_p3	petN-psbM	3	GGCGGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.7	57.6	0.0	0.0	1.734623	30871	31883	1143-1159	1.000000	68.1
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30871	31883	1098-1114	1.000000	68.0
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTTGCTTGGGCG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	8.6	0.0	1.787507	30871	31883	1159-1175	1.000000	67.6
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	47337	48104	906-940	1.000000	77.0
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	47337	48104	905-939	1.000000	77.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.018457	47337	48104	927-961	1.000000	76.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.020450	47337	48104	926-960	1.000000	76.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	47337	48104	877-911	1.000000	76.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.066573	51019	51669	578-830	1.000000	84.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	51019	51669	542-794	1.000000	84.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.200366	51019	51669	566-818	1.000000	83.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GTGAATGGTCGGGATAGCTC	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.220411	51019	51669	588-840	1.000000	83.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGCTGGTAGA	ATGCCTGAAAGTTGGATCGG	20	20	55.0	50.0	58.0	58.2	10.3	0.0	0.247326	51019	51669	555-807	1.000000	83.2
atpB-rbcL_p1	atpB-rbcL	1	TACCGGGCCAATGATTTGAG	GCCTTGAATCCAACACTTGC	20	20	50.0	50.0	57.9	57.9	6.3	0.0	0.121596	57319	58072	864-895	1.000000	82.1
atpB-rbcL_p2	atpB-rbcL	2	GGGGGAAAAGCTACATCCAG	GCCTTGAATCCAACACTTGC	20	20	55.0	50.0	57.9	57.9	12.8	0.0	0.128854	57319	58072	884-915	1.000000	82.0
atpB-rbcL_p3	atpB-rbcL	3	TACCGGGCCAATGATTTGAG	CCAGCCTTGAATCCAACACT	20	20	50.0	50.0	57.9	58.1	6.3	0.0	0.136193	57319	58072	867-898	1.000000	82.0
atpB-rbcL_p4	atpB-rbcL	4	GGGGGAAAAGCTACATCCAG	CCAGCCTTGAATCCAACACT	20	20	55.0	50.0	57.9	58.1	12.8	0.0	0.143452	57319	58072	887-918	1.000000	81.9
atpB-rbcL_p5	atpB-rbcL	5	ATACGCCCCAGGTTTCTTTC	GCCTTGAATCCAACACTTGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.194539	57319	58072	842-873	1.000000	81.6
petA-psbJ_p1	petA-psbJ	1	ATTACGTGTCCAAGGGCTTC	GGCTCATATTCTGGGTTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.122542	66050	67180	1252-1307	1.000000	85.5
petA-psbJ_p2	petA-psbJ	2	GAGAAGGTTCAATTGGCCGA	GGCTCATATTCTGGGTTGGG	20	20	50.0	55.0	58.2	58.0	5.4	0.0	0.191901	66050	67180	1170-1225	1.000000	85.1
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGGCTT	GGCTCATATTCTGGGTTGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.191901	66050	67180	1253-1308	1.000000	84.9
petA-psbJ_p4	petA-psbJ	4	ATTACGTGTCCAAGGGCTTC	TGATCGGTTCAATGGGCATT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.251781	66050	67180	1281-1336	1.000000	84.5
petA-psbJ_p5	petA-psbJ	5	GTCCAAGGGCTTCTGTTCTT	GGCTCATATTCTGGGTTGGG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.291450	66050	67180	1245-1300	1.000000	84.2
psbF-psbE_p1	psbF-psbE	1	AACAGCCAACCATCTCACTG	ATAACTGGCCGTTTCGATCC	20	20	50.0	50.0	58.1	58.1	0.0	16.2	0.148944	67699	67707	117-117	1.000000	79.5
psbF-psbE_p2	psbF-psbE	2	GTGAACAGCCAACCATCTCA	ATAACTGGCCGTTTCGATCC	20	20	50.0	50.0	58.1	58.1	0.0	16.2	0.154315	67699	67707	120-120	1.000000	79.4
psbF-psbE_p3	psbF-psbE	3	TGAACAGCCAACCATCTCAC	ATAACTGGCCGTTTCGATCC	20	20	50.0	50.0	58.1	58.1	0.0	16.2	0.154315	67699	67707	119-119	1.000000	79.4
psbF-psbE_p4	psbF-psbE	4	AACAGCCAACCATCTCACTG	ACCGACAGGGGATTCCATTA	20	20	50.0	50.0	58.1	58.1	0.0	4.8	0.210612	67699	67707	137-137	1.000000	78.9
psbF-psbE_p5	psbF-psbE	5	TGAACAGCCAACCATCTCAC	ACCGACAGGGGATTCCATTA	20	20	50.0	50.0	58.1	58.1	0.0	4.8	0.215983	67699	67707	139-139	1.000000	78.8
ycf2_p1	ycf2	1	ACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAA	20	20	45.0	45.0	56.2	57.2	0.0	0.0	2.600074	92521	92640	240-243	1.000000	66.8
ycf2_p2	ycf2	2	AACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAA	21	20	42.9	45.0	57.0	57.2	0.0	0.0	2.832429	92521	92640	241-244	1.000000	64.9
ycf2_p3	ycf2	3	TCAACGGAGGAACAATATCACC	AGAACCAGAAGCGATGTGAA	22	20	45.5	45.0	58.1	57.2	0.0	0.0	2.970421	92521	92640	243-246	1.000000	63.7
ycf2_p4	ycf2	4	ACGGAGGAACAATATCACCAT	AGAACCAGAAGCGATGTGAA	21	20	42.9	45.0	56.8	57.2	0.0	0.0	3.053608	92521	92640	240-243	1.000000	63.2
ycf2_p5	ycf2	5	ACGGAGGAACAATATCACCA	AGAACCAGAAGCGATGTGAAT	20	21	45.0	42.9	56.2	57.7	0.0	0.0	3.101403	92521	92640	240-243	1.000000	62.8
trnL-CAA-trnV-GAC_p1	trnL-CAA-trnV-GAC	1	GTCTCGCGTGTCTACCATTT	CGGGGGAGCAAGTCAAAATA	20	20	50.0	50.0	58.0	57.9	5.8	0.0	0.130614	96642	98199	1675-1819	1.000000	83.9
trnL-CAA-trnV-GAC_p2	trnL-CAA-trnV-GAC	2	CTCGCGTGTCTACCATTTCA	CGGGGGAGCAAGTCAAAATA	20	20	50.0	50.0	58.0	57.9	5.8	0.0	0.131984	96642	98199	1673-1817	1.000000	83.9
trnL-CAA-trnV-GAC_p3	trnL-CAA-trnV-GAC	3	GTCTCGCGTGTCTACCATTT	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	58.0	57.8	5.8	0.0	0.171648	96642	98199	1614-1758	1.000000	83.6
trnL-CAA-trnV-GAC_p4	trnL-CAA-trnV-GAC	4	CTCGCGTGTCTACCATTTCA	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	58.0	57.8	5.8	0.0	0.173019	96642	98199	1612-1756	1.000000	83.6
trnL-CAA-trnV-GAC_p5	trnL-CAA-trnV-GAC	5	GTCTCGCGTGTCTACCATTT	ATGACTTCCACCACGTCAAG	20	20	50.0	50.0	58.0	57.8	5.8	0.0	0.177663	96642	98199	1613-1757	1.000000	83.5
ndhF-rpl32_p1	ndhF-rpl32	1	AACAAGCAGGAGTCCCAATC	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	57.8	57.7	5.2	0.0	0.511828	109757	111039	986-1398	1.000000	82.3
ndhF-rpl32_p2	ndhF-rpl32	2	GAACAAGCAGGAGTCCCAAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	57.8	57.7	5.2	0.0	0.511828	109757	111039	987-1399	1.000000	82.3
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCAGGAGTCCCAATCCTA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	57.7	57.7	5.2	0.0	0.575686	109757	111039	983-1395	1.000000	81.8
ndhF-rpl32_p4	ndhF-rpl32	4	AGCAGGAGTCCCAATCCTAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	57.5	57.7	5.2	0.0	0.809121	109757	111039	982-1394	1.000000	79.9
ndhF-rpl32_p5	ndhF-rpl32	5	CTTTTTGTTGCCGTCGGAAC	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.5	57.7	17.5	0.0	0.832262	109757	111039	1003-1415	1.000000	79.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGACCGCAGTAAAAGCTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	5.7	0.0	0.148618	111199	112751	1594-1738	1.000000	85.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTGGACCGCAGTAAAAGCTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.148618	111199	112751	1595-1739	1.000000	85.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGGACCGCAGTAAAAGCTTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.0	57.8	5.7	0.0	0.209344	111199	112751	1587-1731	1.000000	84.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGGACCGCAGTAAAAGCTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.209344	111199	112751	1588-1732	1.000000	84.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGGACCGCAGTAAAAGCTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	58.0	57.8	5.7	0.0	0.220635	111199	112751	1592-1736	1.000000	84.5
psaC-ndhE_p1	psaC-ndhE	1	CAGGCTCGTACACATTGAGT	TCAACTCGTATCAATCAATCT	20	21	50.0	33.3	57.6	52.4	0.0	0.0	6.958284	116055	116339	381-386	1.000000	48.1
psaC-ndhE_p2	psaC-ndhE	2	CAATACATCTGTGGGGCAGG	TCAACTCGTATCAATCAATCT	20	21	55.0	33.3	58.3	52.4	0.0	0.0	6.904060	116055	116339	397-402	1.000000	47.8
psaC-ndhE_p3	psaC-ndhE	3	CCAATACATCTGTGGGGCAG	TCAACTCGTATCAATCAATCT	20	21	55.0	33.3	58.3	52.4	0.0	0.0	6.904060	116055	116339	398-403	1.000000	47.8
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	TCAACTCGTATCAATCAATCT	20	21	50.0	33.3	58.1	52.4	0.0	0.0	6.713873	116055	116339	421-426	1.000000	47.4
psaC-ndhE_p5	psaC-ndhE	5	AGCTTTACATCCGTCCCAAG	TCAACTCGTATCAATCAATCT	20	21	50.0	33.3	57.6	52.4	0.0	0.0	6.991255	116055	116339	427-432	1.000000	47.3
ndhA_p1	ndhA	1	GCATAGGGAACATATCGTGGA	GCAAATTCTTCCTCAGCTGG	21	20	47.6	50.0	57.3	57.1	0.0	15.1	2.613537	118433	120598	2336-2394	1.000000	61.2
ndhA_p2	ndhA	2	AGCATAGGGAACATATCGTGG	GCAAATTCTTCCTCAGCTGG	21	20	47.6	50.0	57.3	57.1	0.0	15.1	2.620620	118433	120598	2337-2395	1.000000	61.1
ndhA_p3	ndhA	3	TCCATTCAGCATAGGGAACA	GCAAATTCTTCCTCAGCTGG	20	20	45.0	50.0	56.2	57.1	0.0	15.1	2.730023	118433	120598	2344-2402	1.000000	60.2
ndhA_p4	ndhA	4	TCTCTTGCTTCAGAGAGGTT	GCAAATTCTTCCTCAGCTGG	20	20	45.0	50.0	55.8	57.1	6.7	15.1	3.139558	118433	120598	2289-2347	1.000000	57.0
ndhA_p5	ndhA	5	GCATAGGGAACATATCGTGGAT	GCAAATTCTTCCTCAGCTGG	22	20	45.5	50.0	57.8	57.1	0.0	15.1	3.143372	118433	120598	2336-2394	1.000000	56.9
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	137032	138804	1879-2059	0.733333	71.0
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	137032	138804	1818-1998	0.733333	70.8
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	137032	138804	1822-2002	0.733333	70.7
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	137032	138804	1817-1997	0.733333	70.7
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	137032	138804	1820-2000	0.733333	69.8