Hotspot

ycf1

SSC · 127215-132899 · 5685 bp

Back to Ilex

Pi 0.00498
MarkerSeek score 43.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5685
variable_sites
298
pi
0.00498
unique_haplotype_count
81
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGCACGCCTTCTTGATTTTC ACGAAGGGGACTAAACAGGA 50.0 50.0 58.0 58.0 1160-1184 bp 0.930693 85.9
ycf1_p2 ATTGGGATCGACGTTTTGCT ACGAAGGGGACTAAACAGGA 45.0 50.0 58.2 58.0 1239-1263 bp 0.930693 84.3
ycf1_p3 CGAATCTGATGATCCACCGG ACGAAGGGGACTAAACAGGA 55.0 50.0 58.2 58.0 1178-1202 bp 0.930693 84.2
ycf1_p4 GGCACGCCTTCTTGATTTTC AGGATCCGGACAAAGAAACG 50.0 50.0 58.0 57.9 1093-1108 bp 0.217822 49.9
ycf1_p5 CCGCATTTGACTACGCATTG ACGAAGGGGACTAAACAGGA 50.0 50.0 58.2 58.0 1362-1377 bp 0.217822 48.8