Hotspot

ndhD-psaC

SSC · 120502-120655 · 154 bp

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Pi 0.0356
MarkerSeek score 68.10
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
154
variable_sites
23
pi
0.0356
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 GGAACCTGCAGATATTGGCA AACAACTCGTAGCATGGGTC 50.0 50.0 57.9 57.9 213-220 bp 1.0 90.8
ndhD-psaC_p2 GGAACCTGCAGATATTGGCA TCGTAGCATGGGTCTAGCTT 50.0 50.0 57.9 58.2 207-214 bp 1.0 89.8
ndhD-psaC_p3 GGAACCTGCAGATATTGGCA AGAGGACTGTGTCGGTTGTA 50.0 50.0 57.9 58.0 294-301 bp 1.0 89.1
ndhD-psaC_p4 AGGAACCTGCAGATATTGGC AGAGGACTGTGTCGGTTGTA 50.0 50.0 57.6 58.0 295-302 bp 1.0 86.7
ndhD-psaC_p5 GGAACCTGCAGATATTGGCA AGAGATGTGAATCTGCCTGC 50.0 50.0 57.9 57.7 274-281 bp 0.990099 86.5