Hotspot

rpl32-trnL-UAG

SSC · 116768-117620 · 853 bp

Back to Ilex

Pi 0.0112
MarkerSeek score 62.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
853
variable_sites
97
pi
0.0112
unique_haplotype_count
62
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCTCTTTTGACCGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.9 58.0 808-970 bp 1.0 83.4
rpl32-trnL-UAG_p2 GGGAAATCTCTTTTGACCGGA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.9 58.0 809-971 bp 1.0 83.4
rpl32-trnL-UAG_p3 AGGGAAATCTCTTTTGACCGG AGCGTGTCTACCGATTTCAC 47.6 50.0 57.9 58.0 809-971 bp 1.0 83.4
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTTGACCGG CAGCGTGTCTACCGATTTCA 47.6 50.0 57.9 58.0 810-972 bp 1.0 83.3
rpl32-trnL-UAG_p5 GGGAAATCTCTTTTGACCGGA TCTTCTCGAAGATGGCATGC 47.6 50.0 57.9 58.1 876-1038 bp 0.990099 82.6