Hotspot

ycf3-trnS-GGA

LSC · 46475-47018 · 544 bp

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Pi 0.00583
MarkerSeek score 62.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
544
variable_sites
42
pi
0.00583
unique_haplotype_count
43
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA AAAGGACGGAAAGAGAGGGA 47.6 50.0 58.0 58.0 645-673 bp 1.0 80.7
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 619-647 bp 1.0 80.4
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 590-618 bp 1.0 79.8
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 640-668 bp 1.0 79.7
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA GACGGAAAGAGAGGGATTCG 47.6 55.0 58.0 57.8 641-669 bp 1.0 79.2