Hotspot

petN-psbM

LSC · 30206-31362 · 1157 bp

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Pi 0.0087
MarkerSeek score 70.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1157
variable_sites
102
pi
0.0087
unique_haplotype_count
60
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1249-1356 bp 1.0 77.8
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1188-1295 bp 1.0 76.5
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1249-1356 bp 1.0 73.6
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 1188-1295 bp 1.0 72.3
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 57.1 57.6 1250-1357 bp 1.0 71.8