Hotspot

rpoB-trnC-GCA

LSC · 27894-29152 · 1259 bp

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Pi 0.00782
MarkerSeek score 65.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1259
variable_sites
95
pi
0.00782
unique_haplotype_count
52
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTTCTTCCGTCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.8 58.1 1043-1440 bp 1.0 86.8
rpoB-trnC-GCA_p2 AGTTCTTCCGTCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.7 58.1 1044-1441 bp 1.0 86.8
rpoB-trnC-GCA_p3 CTTCCGTCAAACCCTGATCA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1040-1437 bp 1.0 85.0
rpoB-trnC-GCA_p4 GTTCTTCCGTCAAACCCTGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.8 58.4 1025-1422 bp 1.0 84.7
rpoB-trnC-GCA_p5 AGTTCTTCCGTCAAACCCTG AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.7 58.4 1026-1423 bp 1.0 84.6