Hotspot

rps2-rpoC2

LSC · 17300-17520 · 221 bp

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Pi 0.0121
MarkerSeek score 64.80
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
221
variable_sites
20
pi
0.0121
unique_haplotype_count
60
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 TGCCTCCATCATCTCTTCCA TTTGAGGGGGAAATGAGAGA 50.0 45.0 58.1 55.7 362-372 bp 0.970297 67.5
rps2-rpoC2_p2 AACTCCTGCCTCCATCATCT TTTGAGGGGGAAATGAGAGA 50.0 45.0 58.1 55.7 368-378 bp 0.970297 67.4
rps2-rpoC2_p3 TCCTGCCTCCATCATCTCTT TTTGAGGGGGAAATGAGAGA 50.0 45.0 57.8 55.7 365-375 bp 0.970297 66.9
rps2-rpoC2_p4 GAACTCCTGCCTCCATCATC TTTGAGGGGGAAATGAGAGA 55.0 45.0 57.7 55.7 369-379 bp 0.970297 66.3
rps2-rpoC2_p5 CTCCTGCCTCCATCATCTCT TTTGAGGGGGAAATGAGAGA 55.0 45.0 58.3 55.7 366-376 bp 0.970297 66.1