Hotspot

trnS-GCU-trnG-GCC

LSC · 9118-9794 · 677 bp

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Pi 0.0089
MarkerSeek score 66.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
677
variable_sites
68
pi
0.0089
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ATTAGCAATCCGCCGCTTTA AATCAAACCGAAAGACCCCT 45.0 45.0 58.0 57.0 766-797 bp 1.0 82.9
trnS-GCU-trnG-GCC_p2 ATTAGCAATCCGCCGCTTTA ATCAAACCGAAAGACCCCTT 45.0 45.0 58.0 57.0 765-796 bp 1.0 82.9
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA AATCAAACCGAAAGACCCCT 50.0 45.0 58.2 57.0 815-846 bp 1.0 81.6
trnS-GCU-trnG-GCC_p4 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.2 57.0 814-845 bp 1.0 81.6
trnS-GCU-trnG-GCC_p5 ATTAGCAATCCGCCGCTTTA GAATCAAACCGAAAGACCCCT 45.0 47.6 58.0 58.2 767-798 bp 1.0 81.0