Hotspot

trnK-UUU-rps16

LSC · 4491-5224 · 734 bp

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Pi 0.00848
MarkerSeek score 70.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
734
variable_sites
76
pi
0.00848
unique_haplotype_count
55
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TAGTCTGGACGAATCCCTCC GGTGCTCAACCTACAGGAAC 55.0 55.0 57.9 58.2 913-1042 bp 0.990099 86.8
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 856-985 bp 0.990099 86.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 855-984 bp 0.990099 86.4
trnK-UUU-rps16_p4 TCCTAGCCGCACTTAAAAGC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 871-1000 bp 0.990099 85.6
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 865-994 bp 0.990099 85.5