Hotspot

trnH-GUG-psbA

LSC · 87-516 · 430 bp

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Pi 0.0249
MarkerSeek score 71.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
430
variable_sites
90
pi
0.0249
unique_haplotype_count
65
primer_available
yes
alignment_reliability
0.84
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATGCATGAACGTAACGCTCA 55.0 45.0 58.1 58.3 395-528 bp 1.0 88.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TGCATGAACGTAACGCTCAT 55.0 45.0 58.1 58.3 394-527 bp 1.0 88.7
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA ATGCATGAACGTAACGCTCA 50.0 45.0 57.9 58.3 409-542 bp 1.0 88.7
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TGCATGAACGTAACGCTCAT 50.0 45.0 57.9 58.3 408-541 bp 1.0 88.7
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC TGCATGAACGTAACGCTCAT 50.0 45.0 57.9 58.3 407-540 bp 1.0 88.7