Hotspot

ycf80

Genome · 56414-57868 · 1455 bp

Pi 0.15

MarkerSeek score 83.00

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1455
variable_sites: 526
pi: 0.15
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	AGGGCATGTAACTCAGTGGA	CGTTATCGAGGACCACGTTT	50.0	50.0	58.3	58.0	2741-2879 bp	1.0	89.1
ycf80_p2	AAGGGCATGTAACTCAGTGG	CGTTATCGAGGACCACGTTT	50.0	50.0	57.5	58.0	2742-2842 bp	0.857143	82.2
ycf80_p3	AGGGCATGTAACTCAGTGGA	GCACCTACTATACCTGCAGC	50.0	55.0	58.3	57.8	2527-2582 bp	0.571429	69.1
ycf80_p4	AGGGCATGTAACTCAGTGGA	TGGCACCTACTATACCTGCA	50.0	50.0	58.3	57.8	2529-2584 bp	0.571429	69.0
ycf80_p5	AGGGCATGTAACTCAGTGGA	GTACAAGGTGCAACAGGTCT	50.0	50.0	58.3	57.7	2608-2660 bp	0.571429	68.3