Hotspot

syfB

Genome · 161110-163260 · 2151 bp

Pi 0.18

MarkerSeek score 77.70

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2151
variable_sites: 858
pi: 0.18
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TTGAGGAAAGTCCGGTCTCT	CTCGAAGATGAAGAAGGGGC	50.0	55.0	58.0	58.1	2751 bp	0.142857	45.2
syfB_p2	GGTGCATTAAGAGCGTACCA	CTCGAAGATGAAGAAGGGGC	50.0	55.0	58.0	58.1	2636 bp	0.142857	45.1
syfB_p3	GTGCCACAGAAATAAACCGC	CTCGAAGATGAAGAAGGGGC	50.0	55.0	58.0	58.1	2680 bp	0.142857	45.1
syfB_p4	TTGAGGAAAGTCCGGTCTCT	CGAAGATGAAGAAGGGGCAA	50.0	50.0	58.0	57.9	2749 bp	0.142857	44.7
syfB_p5	GGTGCATTAAGAGCGTACCA	CGAAGATGAAGAAGGGGCAA	50.0	50.0	58.0	57.9	2634 bp	0.142857	44.7