Hotspot

ssrA

Genome · 55811-56205 · 395 bp

Pi 0.139

MarkerSeek score 80.50

Primer available yes

Label ssrA

Feature Metadata

label_name: ssrA
feature_type: gene
region: Genome
length_bp: 395
variable_sites: 124
pi: 0.139
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ssrA_p1	AGGGCATGTAACTCAGTGGA	CACTGATACCTGACATGGCA	50.0	50.0	58.3	57.3	841 bp	0.142857	37.4
ssrA_p2	AAGGGCATGTAACTCAGTGG	CACTGATACCTGACATGGCA	50.0	50.0	57.5	57.3	842 bp	0.142857	36.2
ssrA_p3	AGGGCATGTAACTCAGTGGA	TCACTGATACCTGACATGGC	50.0	50.0	58.3	57.0	842 bp	0.142857	35.1
ssrA_p4	AGGGCATGTAACTCAGTGGA	CACTGATACCTGACATGGCAA	50.0	47.6	58.3	58.0	841 bp	0.142857	34.9
ssrA_p5	GGGCATGTAACTCAGTGGAT	CACTGATACCTGACATGGCA	50.0	50.0	57.3	57.3	840 bp	0.142857	34.4