Hotspot

orf692

Genome · 68643-70721 · 2079 bp

Back to Hypnea

Pi 0.21
MarkerSeek score 78.00
Primer available yes
Label orf692

Feature Metadata

label_name
orf692
feature_type
gene
region
Genome
length_bp
2079
variable_sites
960
pi
0.21
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf692_p1 CGGTTTGATAACGCGATACC AACGACCCGTCAAGAAATCC 50.0 50.0 57.1 58.2 2867-2884 bp 0.428571 56.2
orf692_p2 CGGTTTGATAACGCGATACC CGACCCGTCAAGAAATCCAA 50.0 50.0 57.1 58.2 2865-2882 bp 0.428571 56.2
orf692_p3 TGTTACGGTTTGATAACGCG AACGACCCGTCAAGAAATCC 45.0 50.0 56.9 58.2 2872-2889 bp 0.428571 54.5
orf692_p4 TGTTACGGTTTGATAACGCG CGACCCGTCAAGAAATCCAA 45.0 50.0 56.9 58.2 2870-2887 bp 0.428571 54.5
orf692_p5 TGTTACGGTTTGATAACGCGA AACGACCCGTCAAGAAATCC 42.9 50.0 58.3 58.2 2872-2889 bp 0.428571 52.9