Hotspot

orf193

Genome · 168405-168986 · 582 bp

Back to Hypnea

Pi 0.161
MarkerSeek score 80.30
Primer available yes
Label orf193

Feature Metadata

label_name
orf193
feature_type
gene
region
Genome
length_bp
582
variable_sites
217
pi
0.161
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf193_p1 CATGGGTATTGTTCCTCGGA GTCACTTATGGCATGCAGGA 50.0 50.0 57.0 58.0 1216 bp 0.428571 56.0
orf193_p2 TCATGGGTATTGTTCCTCGG GTCACTTATGGCATGCAGGA 50.0 50.0 57.0 58.0 1217 bp 0.428571 56.0
orf193_p3 CATGGGTATTGTTCCTCGGA TCAACCTTGGCAAGAACGAA 50.0 45.0 57.0 58.0 1383 bp 0.428571 55.8
orf193_p4 CATGGGTATTGTTCCTCGGA TTCAACCTTGGCAAGAACGA 50.0 45.0 57.0 58.0 1384 bp 0.428571 55.8
orf193_p5 TCATGGGTATTGTTCCTCGG TCAACCTTGGCAAGAACGAA 50.0 45.0 57.0 58.0 1384 bp 0.428571 55.8