Hotspot

ntcA

Genome · 158174-158836 · 663 bp

Back to Hypnea

Pi 0.172
MarkerSeek score 79.80
Primer available yes
Label ntcA

Feature Metadata

label_name
ntcA
feature_type
gene
region
Genome
length_bp
663
variable_sites
257
pi
0.172
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ntcA_p1 CTGCCCAGTAGCCAATTTGA TCCCGTCATTCGCCCTTATA 50.0 50.0 58.2 58.3 1884-1887 bp 0.571429 67.3
ntcA_p2 GGTACTGCTTCAGGAACACC TCCCGTCATTCGCCCTTATA 55.0 50.0 58.2 58.3 1918-1921 bp 0.571429 67.0
ntcA_p3 GGTGGTACGTACTTGCTTGT TCCCGTCATTCGCCCTTATA 50.0 50.0 57.8 58.3 1525-1528 bp 0.428571 61.5
ntcA_p4 GGCAGACCATCGGTGAATAG TCCCGTCATTCGCCCTTATA 55.0 50.0 58.1 58.3 1712 bp 0.142857 42.3
ntcA_p5 GGCAGACCATCGGTGAATAG CCCGTCATTCGCCCTTATAA 55.0 50.0 58.1 57.5 1711 bp 0.142857 40.4