Hotspot

psbD-ompR

Genome · 171705-172232 · 528 bp

Back to Hypnea

Pi 0.243
MarkerSeek score 79.40
Primer available yes
Label psbD-ompR

Feature Metadata

label_name
psbD-ompR
feature_type
igs
region
Genome
length_bp
528
variable_sites
256
pi
0.243
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-ompR_p1 GTCGCTCCATGAATAGCACA TGAAGATGACCCAAGCAACC 50.0 50.0 58.1 58.1 1263 bp 0.142857 44.5
psbD-ompR_p2 CAACTCCAAAGCTAGGTGCA TGAAGATGACCCAAGCAACC 50.0 50.0 58.1 58.1 1139 bp 0.142857 44.2
psbD-ompR_p3 TGAACTGGCCAATCCATGAG TGAAGATGACCCAAGCAACC 50.0 50.0 57.9 58.1 811 bp 0.142857 43.9
psbD-ompR_p4 GAACTGGCCAATCCATGAGT TGAAGATGACCCAAGCAACC 50.0 50.0 57.9 58.1 810 bp 0.142857 43.9
psbD-ompR_p5 GTCGCTCCATGAATAGCACA TAGTAAGTCGTGCGGGAGAA 50.0 50.0 58.1 58.2 1390 bp 0.142857 43.8