Hotspot

trnR(acg)-groEL

Genome · 165325-165373 · 49 bp

Back to Hypnea

Pi 0.0807
MarkerSeek score 79.80
Primer available yes
Label trnR(acg)-groEL

Feature Metadata

label_name
trnR(acg)-groEL
feature_type
igs
region
Genome
length_bp
49
variable_sites
10
pi
0.0807
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(acg)-groEL_p1 TGAACATTCCAGTGCGTTCA GATTTGTACCAGCCGCTACA 45.0 50.0 58.0 58.0 976 bp 0.142857 45.2
trnR(acg)-groEL_p2 GATCCACTGAGCTACAAGCC GATTTGTACCAGCCGCTACA 55.0 50.0 58.1 58.0 404 bp 0.142857 45.1
trnR(acg)-groEL_p3 ATACTTGCTGTGCCTTCTGG GATTTGTACCAGCCGCTACA 50.0 50.0 57.9 58.0 762 bp 0.142857 44.6
trnR(acg)-groEL_p4 TTTAAATCGGGCAAGGAGGG GATTTGTACCAGCCGCTACA 50.0 50.0 57.9 58.0 464 bp 0.142857 44.5
trnR(acg)-groEL_p5 TGTGCCTTCTGGTCTCAATG GATTTGTACCAGCCGCTACA 50.0 50.0 57.8 58.0 754 bp 0.142857 44.0