Hotspot

rps1-syfB

Genome · 160666-161109 · 444 bp

Back to Hypnea

Pi 0.0764
MarkerSeek score 81.20
Primer available yes
Label rps1-syfB

Feature Metadata

label_name
rps1-syfB
feature_type
igs
region
Genome
length_bp
444
variable_sites
80
pi
0.0764
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps1-syfB_p1 ACAGGCTGTTGGAAGACCTA TGGTCAGAAGATGGACAAGA 50.0 45.0 58.3 55.8 1221 bp 0.142857 25.8
rps1-syfB_p2 ACAGGCTGTTGGAAGACCTA TGGTCAGAAGATGGACAAGAA 50.0 42.9 58.3 56.6 1221 bp 0.142857 24.1
rps1-syfB_p3 TAACAGGCTGTTGGAAGACC TGGTCAGAAGATGGACAAGA 50.0 45.0 57.4 55.8 1223 bp 0.142857 23.5
rps1-syfB_p4 ACAGGCTGTTGGAAGACCTA ATGGTCAGAAGATGGACAAGA 50.0 42.9 58.3 56.4 1222 bp 0.142857 22.4
rps1-syfB_p5 ACAGGCTGTTGGAAGACCTA TGGTCAGAAGATGGACAAGAAA 50.0 40.9 58.3 57.3 1221 bp 0.142857 21.9