Hotspot

acpP-ycf45

Genome · 112648-112938 · 291 bp

Back to Hypnea

Pi 0.258
MarkerSeek score 78.00
Primer available yes
Label acpP-ycf45

Feature Metadata

label_name
acpP-ycf45
feature_type
igs
region
Genome
length_bp
291
variable_sites
153
pi
0.258
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
acpP-ycf45_p1 GGAGAGGTGGTAGAGTTGGT CGAAACGTAGAGGGTCACAA 55.0 50.0 58.1 57.6 1350-1356 bp 0.428571 63.4
acpP-ycf45_p2 GGAGAGGTGGTAGAGTTGGT ATGAAGCGAAACGTAGAGGG 55.0 50.0 58.1 57.7 1356-1362 bp 0.285714 58.7
acpP-ycf45_p3 AAATCCTGGTCGTGAAGGTG ATGAAGCGAAACGTAGAGGG 50.0 50.0 57.8 57.7 1672-1689 bp 0.285714 58.5
acpP-ycf45_p4 GGAGAGGTGGTAGAGTTGGT GAAGCGAAACGTAGAGGGTC 55.0 55.0 58.1 58.4 1354-1360 bp 0.285714 58.1
acpP-ycf45_p5 CACCCTCTCCGTCCTTCATA ATGAAGCGAAACGTAGAGGG 55.0 50.0 58.2 57.7 1287 bp 0.142857 41.6