Hotspot

trnL(caa)-sufB

Genome · 72849-73690 · 842 bp

Back to Hypnea

Pi 0.194
MarkerSeek score 83.60
Primer available yes
Label trnL(caa)-sufB

Feature Metadata

label_name
trnL(caa)-sufB
feature_type
igs
region
Genome
length_bp
842
variable_sites
363
pi
0.194
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(caa)-sufB_p1 TGCGTATACCAATTTCGCCA TACCCCATGTTGGTTCTTGC 45.0 50.0 58.0 58.1 1099-1125 bp 0.857143 83.9
trnL(caa)-sufB_p2 ACCAATTTCGCCATCTACCC TACCCCATGTTGGTTCTTGC 50.0 50.0 57.9 58.1 1092-1118 bp 0.857143 83.7
trnL(caa)-sufB_p3 TGCGTATACCAATTTCGCCA TAATTCGAGTGGACAGCGTG 45.0 50.0 58.0 58.0 1505 bp 0.142857 45.4
trnL(caa)-sufB_p4 ACCAATTTCGCCATCTACCC TAATTCGAGTGGACAGCGTG 50.0 50.0 57.9 58.0 1498 bp 0.142857 45.2
trnL(caa)-sufB_p5 GAAGGTCTAGCAGACGAAGC TAATTCGAGTGGACAGCGTG 55.0 50.0 58.1 58.0 1820 bp 0.142857 45.0