Hotspot

petB

LSC · 72227-73629 · 1403 bp

Back to Hyparrhenia

Pi 0.00438

MarkerSeek score 59.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1403
variable_sites: 41
pi: 0.00438
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1500-1513 bp	1.0	70.6
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1500-1513 bp	1.0	70.3
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1506-1519 bp	1.0	69.9
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1505-1518 bp	1.0	68.4
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1506-1519 bp	1.0	68.4