Hotspot

rpl14-rpl16

LSC · 78486-78594 · 109 bp

Back to Hyparrhenia

Pi 0.0181

MarkerSeek score 59.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 10
pi: 0.0181
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GATTACCAGCGGCTCCTATG	TAGCTGCCAGTAAAATGCCC	55.0	50.0	57.9	58.2	249-260 bp	1.0	81.8
rpl14-rpl16_p2	ATTACCAGCGGCTCCTATGA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	248-259 bp	1.0	80.3
rpl14-rpl16_p3	TGATTACCAGCGGCTCCTAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	250-261 bp	1.0	80.2
rpl14-rpl16_p4	CGAGCTCCACTGTTATCTGC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.4	58.2	208-219 bp	1.0	79.9
rpl14-rpl16_p5	GATTACCAGCGGCTCCTATG	GCCAGTAAAATGCCCATACG	55.0	50.0	57.9	57.2	244-255 bp	1.0	77.6