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atpI-atpH

LSC · 32415-33236 · 822 bp

Back to Hyparrhenia

Pi 0.0043

MarkerSeek score 58.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 34
pi: 0.0043
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	948-1000 bp	1.0	81.5
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	961-1013 bp	1.0	80.9
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	882-934 bp	1.0	80.9
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	949-1001 bp	1.0	80.6
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	904-956 bp	0.945946	78.0