Hotspot

petN-trnC-GCA

LSC · 18507-18970 · 464 bp

Back to Hyparrhenia

Pi 0.00632

MarkerSeek score 57.80

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 26
pi: 0.00632
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	524-533 bp	1.0	83.3
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	523-532 bp	1.0	83.3
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	522-531 bp	1.0	83.3
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	525-534 bp	1.0	83.2
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	TGCAAATCCTTTACCCCCAG	60.0	50.0	58.3	57.8	579-588 bp	1.0	81.8