Hotspot

trnT-GGU-trnE-UUC

LSC · 15837-16372 · 536 bp

Back to Hyparrhenia

Pi 0.0165

MarkerSeek score 68.80

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 49
pi: 0.0165
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	405-669 bp	1.0	77.9
trnT-GGU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	426-690 bp	1.0	77.7
trnT-GGU-trnE-UUC_p3	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	442-706 bp	1.0	77.6
trnT-GGU-trnE-UUC_p4	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	463-727 bp	1.0	77.5
trnT-GGU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	431-695 bp	1.0	76.9