Hotspot

trnG-UCC-trnT-GGU

LSC · 14045-15764 · 1720 bp

Back to Hyparrhenia

Pi 0.0099
MarkerSeek score 67.90
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1720
variable_sites
117
pi
0.0099
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 1698-2090 bp 1.0 50.1
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1668-2060 bp 1.0 50.0
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT GACTTCTGTCTTACCAAGGCA 38.1 47.6 55.5 57.6 1726-2118 bp 1.0 49.7
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT TGACTTCTGTCTTACCAAGGC 38.1 47.6 55.5 57.6 1727-2119 bp 1.0 49.7
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1664-2056 bp 1.0 49.3