Hotspot

rpoA

LSC · 78253-79260 · 1008 bp

Back to Hyoseris

Pi 0.000992

MarkerSeek score 59.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 1
pi: 0.000992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCAAAACAACTTTTCGGCCT	GCAAGAGAAACAAAGCAAGAGA	40.0	40.9	56.4	57.4	1130 bp	1.0	46.6
rpoA_p2	TCAAAACAACTTTTCGGCCT	AGCAAGAGAAACAAAGCAAGAG	40.0	40.9	56.4	57.4	1131 bp	1.0	46.5
rpoA_p3	TCAAAACAACTTTTCGGCCT	AGCAAGAGAAACAAAGCAAGA	40.0	38.1	56.4	56.3	1131 bp	1.0	45.3
rpoA_p4	TCAAAACAACTTTTCGGCCTT	GCAAGAGAAACAAAGCAAGAGA	38.1	40.9	57.1	57.4	1130 bp	1.0	44.6
rpoA_p5	TTCAAAACAACTTTTCGGCCT	GCAAGAGAAACAAAGCAAGAGA	38.1	40.9	57.1	57.4	1131 bp	1.0	44.6