Hotspot

atpI

LSC · 24520-25263 · 744 bp

Back to Hyoseris

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GTCAAGTCGAGAAAGAGATGGT	AGCCACGCAACATTACCTTA	45.5	45.0	57.8	57.5	871 bp	1.0	58.8
atpI_p2	GTCAAGTCGAGAAAGAGATGGT	TAGCCACGCAACATTACCTT	45.5	45.0	57.8	57.5	872 bp	1.0	58.8
atpI_p3	GTCAAGTCGAGAAAGAGATGGT	TTAGCCACGCAACATTACCT	45.5	45.0	57.8	57.5	873 bp	1.0	58.8
atpI_p4	AGTCAAGTCGAGAAAGAGATGG	AGCCACGCAACATTACCTTA	45.5	45.0	57.6	57.5	872 bp	1.0	56.6
atpI_p5	AGTCAAGTCGAGAAAGAGATGG	TAGCCACGCAACATTACCTT	45.5	45.0	57.6	57.5	873 bp	1.0	56.6