Hotspot

ndhC-trnV-UAC

LSC · 50728-51759 · 1032 bp

Back to Hyoseris

Pi 0.00194
MarkerSeek score 59.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1032
variable_sites
2
pi
0.00194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 1180-1184 bp 1.0 76.7
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1183-1187 bp 1.0 73.7
ndhC-trnV-UAC_p3 TCTGGTCCTTTGCTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1184-1188 bp 1.0 73.7
ndhC-trnV-UAC_p4 TGGTCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1182-1186 bp 1.0 73.7
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.7 58.1 1181-1185 bp 1.0 73.7