Hotspot

trnE-UUC-rpoB

LSC · 12130-13108 · 979 bp

Back to Hyoseris

Pi 0.00102

MarkerSeek score 59.50

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 979
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	1120-1122 bp	1.0	62.3
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	1079-1081 bp	1.0	61.6
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1110-1112 bp	1.0	61.5
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	1115-1117 bp	1.0	61.5
trnE-UUC-rpoB_p5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.0	57.6	1110-1112 bp	1.0	60.9