Hotspot

trnK-UUU-rps16

LSC · 4428-5255 · 828 bp

Back to Hyoseris

Pi 0.00121
MarkerSeek score 59.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
828
variable_sites
1
pi
0.00121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1024-1049 bp 1.0 76.9
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1023-1048 bp 1.0 76.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 964-989 bp 1.0 76.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 963-988 bp 1.0 76.2
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1048-1073 bp 1.0 75.7