Hotspot

ycf1

SSC · 111107-116776 · 5670 bp

Back to Hyoscyamus

Pi 0.33

MarkerSeek score 75.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 1631
pi: 0.33
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACACTCGGAATATTTCATTCGGA	TGGGCGCGTTGGAATTATAA	39.1	45.0	58.0	58.0	421 bp	0.5	35.5
ycf1_p2	ACACTCGGAATATTTCATTCGGA	TTGGGCGCGTTGGAATTATA	39.1	45.0	58.0	58.0	422 bp	0.5	35.5
ycf1_p3	ACACTCGGAATATTTCATTCGGA	ATCGATCTTAACCCAGGCCT	39.1	50.0	58.0	58.2	588 bp	0.5	34.3
ycf1_p4	ACACTCGGAATATTTCATTCGGA	GGATCACCGGGCATATCAAA	39.1	50.0	58.0	57.7	452 bp	0.5	33.7
ycf1_p5	ACACTCGGAATATTTCATTCGG	TGGGCGCGTTGGAATTATAA	40.9	45.0	56.6	58.0	421 bp	0.5	32.4