Hotspot

psaC

SSC · 123202-123447 · 246 bp

Back to Hyoscyamus

Pi 0.528

MarkerSeek score 70.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 93
pi: 0.528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AGGTGCAATTGCTGTAGCTC	AAGAGAAAAACGGAACCCCC	50.0	50.0	58.5	58.0	1644 bp	0.5	55.6
psaC_p2	AGGTGCAATTGCTGTAGCTC	AGTAAAAGGTCCCGGGATGA	50.0	50.0	58.5	58.0	1515 bp	0.5	55.3
psaC_p3	AGGTGCAATTGCTGTAGCTC	AAGCCGCTAAAGTCGCTAAA	50.0	45.0	58.5	57.9	1421 bp	0.5	54.9
psaC_p4	AGGTGCAATTGCTGTAGCTC	CCCCTGCCGTGTACAAAATA	50.0	50.0	58.5	57.9	1628 bp	0.5	54.7
psaC_p5	AGGTGCAATTGCTGTAGCTC	GAGCACAGGTTTGTCGGTAA	50.0	50.0	58.5	58.1	1050 bp	0.5	54.6