Hotspot

ndhI

SSC · 121038-121541 · 504 bp

Back to Hyoscyamus

Pi 0.453

MarkerSeek score 79.20

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 196
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CGATGGGCACAGTGATGTTA	TCCATCTCCTACGGTCCAAA	50.0	50.0	58.0	57.8	1868 bp	0.5	57.9
ndhI_p2	CGATGGGCACAGTGATGTTA	TTCCATCTCCTACGGTCCAA	50.0	50.0	58.0	57.8	1869 bp	0.5	57.9
ndhI_p3	GATGGACTTTACCGAGGCTG	TCCATCTCCTACGGTCCAAA	55.0	50.0	58.1	57.8	1414 bp	0.5	57.6
ndhI_p4	GATGGACTTTACCGAGGCTG	TTCCATCTCCTACGGTCCAA	55.0	50.0	58.1	57.8	1415 bp	0.5	57.6
ndhI_p5	AGATGGACTTTACCGAGGCT	TCCATCTCCTACGGTCCAAA	50.0	50.0	58.1	57.8	1415 bp	0.5	57.0