Hotspot

ndhH

SSC · 117556-118737 · 1182 bp

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Pi 0.505

MarkerSeek score 81.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 519
pi: 0.505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TTGGGAAAACGTCAACGACT	GAAGTCCAACAGGAGCAACA	45.0	50.0	58.0	58.0	1843 bp	0.5	59.5
ndhH_p2	TTGGGAAAACGTCAACGACT	CACCAATGCTAAGATCGGCT	45.0	50.0	58.0	58.0	1798 bp	0.5	59.5
ndhH_p3	TTGGGAAAACGTCAACGACT	ATTCAGGTCCGATACGTTGC	45.0	50.0	58.0	58.1	1603 bp	0.5	59.3
ndhH_p4	TTGGGAAAACGTCAACGACT	GGAGCAACACTTGAAATGGC	45.0	50.0	58.0	57.9	1832 bp	0.5	59.2
ndhH_p5	TTGGGAAAACGTCAACGACT	AAAGGGCCAGCATATTCAGG	45.0	50.0	58.0	57.9	1616 bp	0.5	59.2