Hotspot

ndhF

SSC · 128239-130461 · 2223 bp

Back to Hyoscyamus

Pi 0.465

MarkerSeek score 78.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 854
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGGGCCTATTTCAAAGGAG	ATGGAATCGACCATTTCGCT	50.0	45.0	57.6	57.7	2778 bp	0.5	54.0
ndhF_p2	CCCGTTTGATTCACTGCTCA	ATGGAATCGACCATTTCGCT	50.0	45.0	58.5	57.7	2812 bp	0.5	53.5
ndhF_p3	ATCCCCGTTTGATTCACTGC	ATGGAATCGACCATTTCGCT	50.0	45.0	58.5	57.7	2815 bp	0.5	53.1
ndhF_p4	ACGGGCCTATTTCAAAGGAG	TGAACTGGACAATCAGTGGG	50.0	50.0	57.6	57.4	2577 bp	0.5	52.1
ndhF_p5	ACGGGCCTATTTCAAAGGAG	GAACTGGACAATCAGTGGGT	50.0	50.0	57.6	57.4	2576 bp	0.5	52.1